Mitochondria are known to be as the “powerhouse” of the cells, using the energy obtained from the oxidation of carbohydrates, lipids and amino acids to generate ATP, the universal molecule used by cells to store energy.
In recent years there has been a revolution in the field of mitochondrial physiology through the discovery of new functions performed by these organelles, moving their initial role from simple “powerhouse” to a more complex hub responsible for regulating cellular signaling, energetics and redox balance.
Mitochondria therefore represent a crucial player in the regulation of diverse cellular events (e.g. ATP production, synthesis of phospholipids and heme, calcium homeostasis, ROS production, apoptotic activation and cell death) and the alterations in their functioning are associated with aging and numerous human diseases (like metabolic, endocrine, cardiomyopathies, cancer, diabetes, neurodegeneration and neuromuscular conditions).
The study of mitochondrial function is thus a critical research task that could lead to advancement in mitochondrial related disease management and treatments.
an innovative in vitro platform dedicated to the characterization of mitochondrial function and dysfunction in physiopathologically relevant scenarios
a review of the assays provided with our platform
Total Thiol assay is used to determine the antioxidant capacity of a cells.
Aconitase activity is used as a biomarker for oxidative damage.
CS activity is used to evaluate the mitochondrial content.
Analyses of the oxidative phosphorylation system is used for the characterization of mitochondrial respiratory capacity.
Complex I+III activity is used to evaluate the activity of the first complex of the mitochondrial electron transfer chain.
mGPDH: Cyt C oxido-reductase activity is used to determine the activity of mytochondrial Glycerol-3-phosphate dehydrogenase.
Proline:Cyt C oxido-reductase activity is used to determine the activity of Proline dehydrogenase.
Succinate:Cyt C oxido-reductase activity is used to determine the activity of Succinate dehydrogenase, the respiratory Complex II.
Cytochrome c oxidase (COX) activity is used to determine the activity of the respiratory Complex IV and can represent an indicator of the oxidative capacity of the cells..